Direct evaluation of radiation damage in human hematopoietic progenitor cells in vivo

Journal Article · Sat Jan 01 04:00:00 EST 1994 · Radiation Research

DOI:https://doi.org/10.2307/3578793· OSTI ID:45268

Kyoizumi, Seishi; McCune, J M; Namikawa, Reiko ^[1]

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1. Systemix Inc., Palo Alto, CA (United States)

We have developed techniques by which normal functional elements of human bone marrow can be implanted into immunodeficient C.B-17 scid/scid (SCID) mice. Afterward, long-term multilineage human hematopoiesis is sustained in vivo. We evaluated the effect of irradiation on the function of human bone marrow with this in vivo model. After whole-body X irradiation of the engrafted animals, it was determined that the D{sub 0} value of human committed progenitor cells within the human marrow was 1.00 {+-} 0.09 (SEM) Gy for granulocyte-macrophage colony-forming units (CFU-GM) and 0.74 {+-} 0.12 Gy for erythroidburst-forming units (BFU-E). The effects of irradiation on the hematopoietic elements were reduced when the radioprotective agent WR-2721 was administered prior to irradiation. After low-dose irradiation, recovery of human granulocyte colony-stimulating factor (G-CSF). This small animal model may prove amenable for the analysis of the risk of the exposure of humans to irradiation as well as for the development of new modalities for the prevention and treatment of radiation-induced hematopoietic damage. 41 refs., 5 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 45268
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 137; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
BONE MARROW
CFU
DAMAGE
LOW DOSE IRRADIATION
MICE
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
X RADIATION