A YAC contig encompassing the XRCC5 (Ku80) DNA repair gene and complementation of defective cells by YAC protoplast fusion
- Univ. of Sussex, Brighton (United Kingdom); and others
The Chinese hamster ovary xrs mutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33-q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred to xrs cells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines. 44 refs., 2 figs., 4 tabs.
- OSTI ID:
- 446977
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 30; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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Journal Article
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Tue Apr 11 00:00:00 EDT 1995
· Proceedings of the National Academy of Sciences of the United States of America
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Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen
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Mon Jan 30 23:00:00 EST 1995
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Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL MARKERS
CHO CELLS
CONTIGS
DNA
DNA REPAIR
DNA-CLONING
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HUMAN CHROMOSOME 2
HYBRIDIZATION
IONIZING RADIATIONS
MUTANTS
PHENOTYPE
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RADIOSENSITIVITY
STRAND BREAKS
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL MARKERS
CHO CELLS
CONTIGS
DNA
DNA REPAIR
DNA-CLONING
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HUMAN CHROMOSOME 2
HYBRIDIZATION
IONIZING RADIATIONS
MUTANTS
PHENOTYPE
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RADIOSENSITIVITY
STRAND BREAKS