Automated linkage analysis in psychiatric disorders
- Royal Edinburgh Hospital (United Kingdom); and others
A genome-wide search for linkage of microsatellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure. We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein-labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP.) The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager{trademark} software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs. The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic-depressive illness is discussed. 14 refs., 3 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 443840
- Journal Information:
- American Journal of Medical Genetics, Vol. 60, Issue 3; Other Information: PBD: 19 Jun 1995
- Country of Publication:
- United States
- Language:
- English
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INCLUDING NUCLEAR AND PARTICLE DETECTORS
99 MATHEMATICS
COMPUTERS
INFORMATION SCIENCE
MANAGEMENT
LAW
MISCELLANEOUS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOMES
GENETIC MAPPING
COMPUTER CODES
GENES
DNA SEQUENCING
HEREDITARY DISEASES
MENTAL DISORDERS
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AUTOMATION
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ACCURACY
BIOLOGICAL MARKERS
ELECTROPHORESIS
POLYMERASE CHAIN REACTION
GENOTYPE
MAN