Assessing sequential oncogene amplification in human breast cancer

Journal Article · Fri Sep 01 04:00:00 EDT 1995 · Cytometry

DOI:https://doi.org/10.1002/cyto.990210106· OSTI ID:443396

Janocko, L E; Lucke, J F; Groft, D W; Brown, K A ^[1]

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1. Allegheny-Singer Research Inst., Pittsburgh, PA (United States); and others

Studies of amplification and/or overexpression of c-myc, HER-2/neu, and H-ras in breast cancer have shown that each is associated with a poor prognosis. The purpose of this study was to explore the possibility that there is a preferred sequence of amplification of these oncogenes in breast cancer. The frequencies of amplification and patterns of co-amplification of c-myc, HER-2/neu, and H-ras were studied in a group of 84 breast cancers. The data suggested a preferred sequence of amplification that consisted of c-myc amplification-HER-2/neu amplification-H-ras amplification. This model was supported by loglinear analysis. In addition, the levels of amplification of JC-A, a DNA fragment newly isolated from a patient with advanced breast cancer, were studied in 61 of these cases. The data suggested that JC-A amplification occurred early. Loglinear analysis supported a model in which JC-A amplification occurred either before or after c-myc amplification but was unrelated to Her-2/neu or ras amplification. 35 refs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 443396
• Journal Information: Cytometry, Journal Name: Cytometry Journal Issue: 1 Vol. 21; ISSN CYTODQ; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL EVOLUTION
GENE AMPLIFICATION
GENE REGULATION
GENETICS
MAMMARY GLANDS
NEOPLASMS
ONCOGENES
PATIENTS
STATISTICS