Preferred genetic evolutionary sequences in human breast cancer: A case study

Journal Article · Fri Sep 01 04:00:00 EDT 1995 · Cytometry

DOI:https://doi.org/10.1002/cyto.990210104· OSTI ID:443394

Shackney, S E; Smith, C A; Pollice, A A ^[1]

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1. Allegheny-Singer Research Inst., Pittsburgh, PA (United States); and others

Multiparameter flow cytometry studies were performed on the cells of an aggressive human breast cancer at the time of diagnosis and at relapse. The aneuploid cells that overexpressed large amounts of both HER-2/neu and ras survived intensive chemotherapy and were responsible for tumor relapse. At relapse, these cells were shown to overexpress simultaneously at least five oncogenes: HER-2/neu, ras, EGF receptor, p53 and c-myc. A partial reconstruction of the genetic evolutionary sequence in this tumor indicated that HER-2/neu overexpression was an early step in the sequence. Subsequent HER-2/neu overexpression, EGF receptor overexpression and p53 protein overexpression were each associated with ras overexpression. The data suggest that ploidy and oncogene overexpression cannot be used as independent clinical prognostic factors. The ability to characterize tumors according to the degree of advancement in the genetic evolutionary might serve as a basis for genetic staging for adjuvant therapy. 5 refs., 5 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 443394
• Journal Information: Cytometry, Journal Name: Cytometry Journal Issue: 1 Vol. 21; ISSN CYTODQ; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANEUPLOIDY
CELL FLOW SYSTEMS
CHEMOTHERAPY
DNA SEQUENCING
GENE MUTATIONS
GENE REGULATION
GENETICS
MAMMARY GLANDS
NEOPLASMS
ONCOGENES
PATIENTS
POST-IRRADIATION THERAPY
TUMOR CELLS