Hydroxamate recognition during iron transport from hydroxamate-iron chelates
Journal Article
·
· J. Bacteriol., v. 115, no. 3, pp. 912-918
OSTI ID:4421953
Kinetics of radioactive iron transport from three structurally different secondary hydroxandate-iron chelates (schizokineniron, produced by Bacillus megaterium ATCC 19213; Desferaliron, produced by an actinomycete; and aerobactin- iron, produced by Aerobacter aerogenes 62-1) revealed that B. megateriurm SK 11 (a mutant that cannot synthesize schizokinen) has a specific transport system for utilization of ferric hydroxamates with a recognition capacity based on the chemical structure of the hydroxamate. Both Desferal and schizokinen enhanced iron uptake in this organism; however, Desferal-iron delivered only one-sixth the level of iron incorporated from the schizokinen-iron chelate. Desferaliron did not generate the rapid rates of iron transport noted with schizokinen-iron at elevated iron concentrations. Assays containing large excesses of either iron- free Desferal or iron-free schizokinen suggested that the iron-free hydroxamate may compete with the ferric hydroxamate for acceptance by the transport system although the system has greater affinity for the iron chelate. Aerobactin-iron did not stimulate iron uptake in B. megaterium SK 11 and aerobactin inhibited growth of this organism, indicating that B. megateriuni SK 11 cannot efficiently process the aerobactin-iron chelate. (auth)
- Research Organization:
- Univ. of Mississippi, Jackson
- NSA Number:
- NSA-29-002960
- OSTI ID:
- 4421953
- Journal Information:
- J. Bacteriol., v. 115, no. 3, pp. 912-918, Journal Name: J. Bacteriol., v. 115, no. 3, pp. 912-918; ISSN JOBAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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