Biochemical studies on the x mutation of bacteriophage T4: differential inhibition of x$sup +$ and x DNA synthesis by mitomycin C

Journal Article · Tue Jan 01 04:00:00 EST 1974 · J. Virol., v. 13, no. 1, pp. 1-8

OSTI ID:4410207

Shimizu, K; Sekiguchi, M

Biochemical studies were carried out to determine the effect of x mutation on T4 DNA synthesis. The rate and final extent of DNA synthesis are almost the same with T4D- and T4x-infected cells, although the burst size of T4x is about one-sixth that of the wild type. The DNA synthesis of T4x-infected cells is more readily inhibited by mitomycin C than is that of T4 wild type. When mitomycin C was added during active phage growth, DNA synthesis of T4x halted almost immediately. T4 DNA polymerases isolated from x/sup +/- and x- infected cells, however, exhibit no difference with regard to their sensitivities to mitomycin C, priming activities with alkylated or ultraviolet light-irradiated templates and other enzymatic properties. (auth)

• Research Organization: Kyushu Univ., Fukuoka
• NSA Number: NSA-29-010362
• OSTI ID: 4410207
• Journal Information: J. Virol., v. 13, no. 1, pp. 1-8, Journal Name: J. Virol., v. 13, no. 1, pp. 1-8; ISSN JOVIA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*BACTERIOPHAGES-- BIOCHEMICAL REACTION KINETICS
*DNA-- BIOSYNTHESIS
BIOLOGICAL RADIATION EFFECTS
MITOMYCIN
MUTATIONS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOTON BEAMS
POLYMERASES
ULTRAVIOLET RADIATION