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Characterization of a bacteriophage T4 mutant lacking DNA-dependent ATPase

Journal Article · · J. Virol., v. 15, no. 1, pp. 50-54
OSTI ID:4177059
A DNA-dependent ATPase has previously been purified from bacteriophage T4-infected Escherichia coli. A mutant phage strain lacking this enzyme has been isolated and characterized. Although the mutant strain produced no detectable DNA-dependent ATPase, growth properties were not affected. Burst sizes were similar for the mutant phage and T4D in polAl, recB, recC, uvrA, uvrB, uvrC, and various DNA-negative E. coli. UV sensitivity and genetic recombination were normal in a variety of E. coli hosts. Mapping data indicate that the genetic locus controlling the mutant occurs near gene 56. The nonessential nature of this gene is discussed. (auth)
Research Organization:
Univ. of Western Ontario, London
NSA Number:
NSA-33-005723
OSTI ID:
4177059
Journal Information:
J. Virol., v. 15, no. 1, pp. 50-54, Journal Name: J. Virol., v. 15, no. 1, pp. 50-54; ISSN JOVIA
Country of Publication:
United States
Language:
English