Effect of irradiation on the biochemical changes of mammalian cells in the absence and presence of particulate materials. Final report
The effect of x irradiation on the phagocytic aspects of host defense was investigated. Leukocytes collected from x-irradiated subjects (human and animal), in general, have altered phagocytic, metabolic, enzymatic, and bactericidal activities. The alterations in these activities can be reversed by the administration of bacterial endotoxin. Lead shielding and/or /sup 3/H- thymidine uptake experiments indicate that the majority of the radiosensitive cells originate in the sternum marrow. Cell synchronization studies with the administration of rabbit antineutrophilic serum (ANS) to guinea pigs suggest that the radiosensitive cells are probably multipotential cells. X irradiation of leukocytes in vitro (up to 50,000 R) have resulted in no demonstrable changes in the phagocytosis-associated activities. However, leukocytes subjected to x irradiation (1000 R) and phagocytosis concurrently have increased phagocytic and bactericidal activities. These stimulations are reversed by the addition -of catalase or SH compounds. It appears that the phagocytosisassociated H/sub 2/O/ sub 2/ production is involved in the antimicrobial activities of these cells. During this period significant information has become available regarding the mechanism of synergistic antimicrobial activity of H/sub 2/O/sub 2/, peroxidase (or catalase), and a halide in leukocytes collected from x irradiated and control subjects. (auth)
- Research Organization:
- Saint Margaret's Hospital, Boston, Mass. (USA)
- NSA Number:
- NSA-29-010393
- OSTI ID:
- 4404869
- Report Number(s):
- TID--26509
- Country of Publication:
- United States
- Language:
- English
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