Cell cycle of matrix cells in the mouse embryo during histogenesis of telencephalon
Pregnant female mice were injected intraperitoneally with 5 mu Ci/g body weight of /sup 3/H-thymidine (spec. act. 12 mu Ci/mM) at 1:30 p.m. on day 10, 13, or 17 of gestation and were put to death at 1 or 2 hr intervals per group. Embryos were removed quickly from mothers and fixed in Bouin's solution. The prepared slides were observed microscopically. The duration of the cell cycle of the matrix cells of the telencephalon was determined by direct graphic measurement, plotting the percentage of labeled mitosis against the time after / sup 3/H-thymidine injection according to the method of Quastler and Sherman. The total cell cycle times in day 10, 13, and 17 groups were 7.0, 15.5, and 26.0 hr, respectively. It was characteristic in the alteration of cell cycle of matrix cells in the telencephalon during mouse embryonic life that not only G/sub 1/ but also S phase lengthened linearly with embryonic age, and both G/sub 2/ and M phases remained constant. According to these facts, the matrix cells seemed to change cytogenetically with increase of age so as to produce different neurons that would progressively make up different layers in the neocortex. (JA)
- Research Organization:
- Nagoya Univ., Japan
- NSA Number:
- NSA-29-010550
- OSTI ID:
- 4402892
- Journal Information:
- Nagoya Daigaku Kankyo Igaku Kenkyusho Nempo, v. 20, pp. 103-106, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: JA (JA)
- Country of Publication:
- Japan
- Language:
- English
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