Tritium labeling of polyribonucleotides (in German)
Thesis/Dissertation
·
OSTI ID:4398790
Polyribonucleotides were labeled with tritium during incubation in tritium-contained water by an exchange reaction. A specific activity between 1 and 16 mli/mMol was obtained with pure polyribonucleotides: with increasing reaction time the polynucleotides decompose, the bases remain chemically unchanged. The chain shortening of the pure polyribonucleotide cannot be traced back to the BETA -radiation of tritium. Iodized cytidine diphosphate was chiefly labelled in position 5 with a specific activity of 30 mli/ mMol. In the enzymatic polymerization of 3-H-CDP to 3-H-poly(i), an isotope effect occurs that lowers the specific activity of the polymer by up to 20%. (GE)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-29-000209
- OSTI ID:
- 4398790
- Country of Publication:
- Germany
- Language:
- German
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Related Subjects
*ADENYLIC ACID-- LABELLING
*CYTIDYLIC ACID-- LABELLING
*TRITIUM-- RADIOCHEMISTRY
*URIDYLIC ACID-- LABELLING
ISOTOPE EFFECTS
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
N48830 --Life Sciences--New Tracer Techniques--Other
ORGANIC POLYMERS
RNA
STRAND BREAKS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
*CYTIDYLIC ACID-- LABELLING
*TRITIUM-- RADIOCHEMISTRY
*URIDYLIC ACID-- LABELLING
ISOTOPE EFFECTS
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
N48830 --Life Sciences--New Tracer Techniques--Other
ORGANIC POLYMERS
RNA
STRAND BREAKS
TRACER TECHNIQUES
TRITIUM COMPOUNDS