Comparative studies on fast neutron and gamma ray-induced mutation and germination rates in barley (Hordeum vulgae L.) (in German)
>The effects of various modifying factors were studied on fast neutron- and gamma -induced mutation and germination rates in barley. The water content of dormant seeds and the oxygen concentration during and after irradiation affect radiosensitivity. Only the water content influences the effects of fast neutrons. The degree of imbibition of seeds very markedly affects their radiosensitivity. Increased soaking causes gamma radiation to produce a greater reduction in survival rate, chlorophyll mutation frequency, and fertility than fast neutrons. Soaking, with subsequent drying, increases the radiation effects of both. Soaking in 0.1 m cystein very markedly irfluences the germination of neutron- or gamma -irradiated barley seeds, but affects the chlorophyll mutation rate only slightly. The survival rate of gamma -irradiated seeds goes up with increasing imbibition. Soaking in 0.1 m cystein, with subsequent drying, increases the germination of barley seeds, following gamma - but not fast-neutronirradiation. Cystein does not appear to affect the chlorophyll mutation rate. No differences were observed in the chlorophyll mutation spectrum of neutron- and gamma - irradiated seeds. Seeds treated with fast neutrons or ethyl methanesulfonate (EMS) showed no differences in macromutation frequency, but double the chlorophyll mutation frequency was obtained with EMS. Of 15 mutants selected for early ripening, 9 proved genuine. The two Erectoides mutants tested showed clear differences in stalk and spike length, but no significant difference in yield between the mutant and the original Union strain could be established. (auth)
- Research Organization:
- Hochschule fuer Bodenkultur, Vienna (Austria)
- NSA Number:
- NSA-29-007935
- OSTI ID:
- 4366115
- Report Number(s):
- INIS-mf-112
- Resource Relation:
- Other Information: Orig. Receipt Date: 30-JUN-74
- Country of Publication:
- Austria
- Language:
- German
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