Physiological and spectrophotometric analysis of irradiated tissue culture cells (in German)
Book
·
OSTI ID:4358810
According to the opinion of the author, investigations in radiobiology should nowadays not be restricted to the study and determination of radiation damages as a consequence of complex reactions, but rather be carried out in order to determine and elucidate single events and reactions. A number of problems in radiobiology are discussed in order to permit further investigations to be made with respect to tissue culture techniques, cytologic-cytochemical aspects, radiation genetics, and molecular biological aspects. The main emphasis is placed on the study of mammalian tissue culture cells and radioinduced interactions with the biological object. Investigations on population dynamics, colony size distribution, and karyological behavior of stemline cultures and single cells, and uv-microspectrophotometric measurements on living cells have been carried out. Furthermore, a method for the calculation of the uv-energy- abgorbed by living cells during irradiation is given and the performance and results of investigations for the determination of damaging effects in cells induced by uv-light and x-ray irradiation, the cytophotometric determination of the nucleic acid content of irradiated and unirradiated cells, and the molecule modifying effect of uvlight on the DNA synthesis are described. (GE)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-29-015847
- OSTI ID:
- 4358810
- Country of Publication:
- Germany
- Language:
- German
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Related Subjects
*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*BIOLOGICAL RADIATION EFFECTS-- BIOCHEMICAL REACTION KINETICS
*TISSUE CULTURES-- RADIOBIOLOGY
DNA
GENETICS
MOLECULAR BIOLOGY
N48200* --Life Sciences--Radiation Effects on Cells
NUCLEIC ACIDS
PHOTON BEAMS
PHYSIOLOGY
POPULATION DYNAMICS
RADIATION INJURIES
SPECTROPHOTOMETRY
ULTRAVIOLET RADIATION
X RADIATION
*BIOLOGICAL RADIATION EFFECTS-- BIOCHEMICAL REACTION KINETICS
*TISSUE CULTURES-- RADIOBIOLOGY
DNA
GENETICS
MOLECULAR BIOLOGY
N48200* --Life Sciences--Radiation Effects on Cells
NUCLEIC ACIDS
PHOTON BEAMS
PHYSIOLOGY
POPULATION DYNAMICS
RADIATION INJURIES
SPECTROPHOTOMETRY
ULTRAVIOLET RADIATION
X RADIATION