In vivo study of the relative radiosensitivity of oxic and hypoxic tumor cells in the solid Sarcoma 180 after irradiation with high or low LET. External measurements using labeled iododeoxyuridine

Book · Mon Jan 01 04:00:00 EST 1973

OSTI ID:4358529

Porschen, W; Piepenbring, W; Muehlensiepen, H; Feinendegen, L E

The relative radiosensitivity of oxic and hypoxic tumor cells was analyzed using an in-vivo measuring method. The DNA of the tumor was labelled in vivo with /sup 125/I or /sup 131/I-iododeoxyuridine that can easily be externally measured for long periods. About 10/sup 6/ tumor cells were injected into the thigh of the experimental animals, NMRI mice. The growing tumors were locally exposed to 2,000 or 4,000 R min of gamma radiation from a /sup 137/Cs source, with a dose rate of 100 R; or with l5-MeV neutrons from a neutron generator of the type Dynagen 300, with a dose of 300 or 500 rads. After irradiation, an increase in cell loss was observed. This increase was found to depend on the dose, the type of irradiation, and the time interval between the labelling and the irradiation. The tumor cells irradiated under hypoxic conditions showed, as expected, a reduction of the radiosensitivity. The experimental technique is well suited to the in-vivo determiantion of the relative radiosensitivity of hypoxitic tumor cells. The data obtained by in-vivo experiments agree with those obtained by in-vitro experiments. (GE)

• Research Organization: Kernforschungsanlage, Juelich, Ger.
• NSA Number: NSA-29-015861
• OSTI ID: 4358529
• Country of Publication: Germany
• Language: English

Related Subjects

*TUMOR CELLS-- RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
DEOXYURIDINE
DNA
DOSE RATES
GAMMA RADIATION
IN VIVO
IODINE 125
IODINE 131
LABELLING
MICE
N48200* --Life Sciences--Radiation Effects on Cells
N48520 --Life Sciences--Radiation Effects on Animals--Vertebrates
NEUTRONS
PHOTON BEAMS
RADIATION DOSES
RESPONSE MODIFYING FACTORS
SARCOMAS