Effect of fast electrons with different energy spectra on two DNA fractions of meristematic cells

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Biophysik, v. 10, no. 2, pp. 157-162

OSTI ID:4350205

Elmshaeuser, H J; Markus, B

In previous observations of the frequency of chromosome aberrations in anaphase cell of Alliuan cepa irradiated with fast electrons in different depths, the varying radiosensitivity in different stages of the cell cycle could be interpreted by assuring a different effect in euchromatin and heterochromatin synthesized at different times. In the experiments described here, two DNA fractions of different molecular weight extracted from the same object and from Vicia faba and defined as euchromatin and heterochromatin by the uptake of /sup 3/ H-actinomycin D were irradiated under the same conditions. The change in uv absorption after surface and depth irradiation was different for the two chromatin fractions and can be explained in accordance with the chromosome aberrations by repair of euchromatin but not of heterochromatin 9 h after irradiation. (auth)

• Research Organization: Univ., Goettingen, Ger.
• NSA Number: NSA-29-015835
• OSTI ID: 4350205
• Journal Information: Biophysik, v. 10, no. 2, pp. 157-162, Journal Name: Biophysik, v. 10, no. 2, pp. 157-162; ISSN BPYKA
• Country of Publication: Germany
• Language: German

Related Subjects

*CHROMATIN-- BIOLOGICAL RADIATION EFFECTS
*CHROMOSOMAL ABERRATIONS-- BIOLOGICAL REPAIR
*HETEROCHROMATIN-- BIOLOGICAL RADIATION EFFECTS
ACTINOMYCIN
ALLIUM CEPA
DNA
ELECTRON BEAMS
IRRADIATION
N48130* --Life Sciences--Radiation Effects on Biochemicals-- In Plants
N48410 --Life Sciences--Radiation Effects on Plants--Basic Studies
TRITIUM COMPOUNDS
ULTRAVIOLET SPECTRA
VICIA