Studies on lysosomes after irradiation. I. A quantitative histochemical method for the study of lysosomal membrane permeability and acid phosphatase activity

Journal Article · Fri Mar 01 04:00:00 EDT 1974 · Radiat. Res., v. 57, no. 3, pp. 403-415

DOI:https://doi.org/10.2307/3573888· OSTI ID:4345799

Aikman, A A; Wills, E D

A simple histochemical method was developed which enables the permeability of lysosomal membranes to be studied semiquantitatively and the relative specific activity of lysosomal acid phosphatase to be measured quantitatively in tissue sections after irradiation. Increase in membrane permeability, but not of enzyme activity, occurs after treatment of tissue sections with solutions of low pH or injection of vitamin A into the animal. Enzyme activity measured by the method has been related to standard determinations of tissue homogenates. (auth)

• Research Organization: St. Bartholomew's Hospital, London
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-026965
• OSTI ID: 4345799
• Journal Information: Radiat. Res., v. 57, no. 3, pp. 403-415, Journal Name: Radiat. Res., v. 57, no. 3, pp. 403-415; ISSN RAREA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*LYSOSOMES-- PERMEABILITY
BIOLOGICAL RADIATION EFFECTS
CATALYSIS
MEMBRANES
METABOLISM
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
PH VALUE
RADIATIONS
TISSUES
VITAMIN A