Defense of cutaneous cells against uv irradiation. I. Photomediated binding of trimethyl psoralen to DNA of melanoma cells in culture

Journal Article · Tue May 01 00:00:00 EDT 1973 · J. Invest. Dermatol., v. 60, pp. 270-273

DOI:https://doi.org/10.1111/1523-1747.ep12722972· OSTI ID:4345709

Carter, D M; McMacken, M d.V.; Condit, E S

Pigmented and nonpigmented murine melanoma cells in culture were irradiated with ultraviolet light (365 nm) in the presence of tritiated trimethyl psoralen (/sup 3/H TMP) and cellular DNA was extracted. For each cell type, regardless of the presence of pigment, the rates of covalent binding of /sup 3/H TMP to DNA and the saturation points reached were the same. The formation by irradiation of both monofunctional and bifunctional (crosslinking) adducts of TMP with cellular DNA was demonstrated by the resistance to enzymic and chemical hydrolysis of the DNA-/sup 3/H TMP complex and by the pattern of elution of extracted DNA from hydroxyapatite columns. These studies suggest that packaged intracellular melanin pigment has limited ability to protect cellular DNA from this type of photomediated injury. (auth)

• Research Organization: Yale Univ. School of Medicine, New Haven
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-026983
• OSTI ID: 4345709
• Journal Information: J. Invest. Dermatol., v. 60, pp. 270-273, Other Information: Orig. Receipt Date: 30-JUN-74
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

N48210* -Life Sciences-Radiation Effects on Cells- External Source
*ANIMAL CELLS- BIOLOGICAL RADIATION EFFECTS
*DNA- BIOLOGICAL RADIATION EFFECTS
*MELANIN- RADIOSENSITIVITY EFFECTS
*PSORALEN- BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CROSS-LINKING
HYDROLYSIS
MICE
PHOTON BEAMS
RADIATION PROTECTION
SKIN
TRITIUM
ULTRAVIOLET RADIATION RADIOSENSITIVITY EFFECTS