Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase
Journal Article
·
· Analytical Letters
- Lund Univ. (Sweden)
A Sequential Injection (SI) system was used to analyze the ethanol concentration in fermentation broth. The method is based on the use of immobilized NAD{sup +} dependent alcohol dehydrogenase. A non-linear standard curve for ethanol (range 0.25-100 mM) was used to determine the concentration in fermentation broth and the results correlated well with HPLC measurements. The assay time was 140 s, 0.5 {mu}mol of cofactor was used for each determination, and the relative standard deviation was less than 6% when analyzing fermentation samples. The assay system is very stable and makes it possible to reduce the cofactor consumption while keeping the system set up simple.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 433460
- Journal Information:
- Analytical Letters, Vol. 29, Issue 7; Other Information: PBD: May 1996
- Country of Publication:
- United States
- Language:
- English
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