Respiration, growth, and viability of repair-deficient mutants of Escherichia coli after ultraviolet irradiation

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Int. J. Radiat. Biol., v. 25, no. 1, pp. 51-60

DOI:https://doi.org/10.1080/09553007414550051· OSTI ID:4332535

Swenson, P A; Schenley, R L

E scherichia coli WP2 (a tryptophan-requiring strain of B/r) and three mutants deficient in DNA repair processes were used for a comparative study of the effects of ultraviolet radiations on respiration. An hcr- strain behaved similarly to WP2 hcr/sup +/; respiration ceased about an hour after uv. The doses used for stopping respiration were 55 and 520 eng/mm/sup 2/ respectively; these doses reduced survival in each case to about 0.5% WP2 rec- (Rec A) and WP2 exrstrains did not cease respiring after receiving a range of doses up to 520 ergs/mm/sup 2/. Following irradiation, WP2 hcr/sup +/ and WP2 hcrcells degraded their DNA slightly if at all, but degradation was extensive in the rec/sup -/ and erx/sup -/ strains at doses that reduced survival to 1.0% (33 and 150 ergs/mm/sup 2/ respectively). It was concluded that cessation of respiration of irradiated Escherichia coli is dependent on the integrity of their DNA and not on the radiation sensitivity. (auth)

• Research Organization: Oak Ridge National Lab., TN
• NSA Number: NSA-29-029699
• OSTI ID: 4332535
• Journal Information: Int. J. Radiat. Biol., v. 25, no. 1, pp. 51-60, Journal Name: Int. J. Radiat. Biol., v. 25, no. 1, pp. 51-60; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*RESPIRATION-- BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
DECOMPOSITION
DNA
DOSE- RESPONSE RELATIONSHIPS
GROWTH
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
RADIOSENSITIVITY
ULTRAVIOLET RADIATION