Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody
Viral antigens were fixed to the surface of microtiter wells, and serial dilutions of antiviral antibody were added. The amount of antiviral antibody bound to viral antigens was determined by measuring the extent to which the antiviral antibody either inhibited the specific binding of /sup 131/I-labeled antiviral immunoglobulin G (IgG) (direct technique) or enhanced the speciftc binding of /sup 125/I-labeled anti-IgG (indirect technique). Immune complexes composed of viral antigens and antiviral antibody (human) could be detected by the binding of /sup 125/I-labeled rheumatoid factor. Specific binding was influenced by the concentration of protein in the diluents used during the different steps of the procedure. A high concentration of protein in the diluent used with the viral antigens decreased specific binding, whereas a high concentration of protein in the diluent used with /sup 125/I-labeled anti-IgG increased specific binding by decreasing nonspeciftc attachment of the labeled anti-IgG. Under the conditions employed, the titer of a given antiviral serum was several hundredfold greater by the indirect than by the direct technique. (auth)
- Research Organization:
- National Inst. of Dental Research, Bethesda, MD
- NSA Number:
- NSA-29-024575
- OSTI ID:
- 4331079
- Journal Information:
- Appl. Microbiol., v. 25, no. 4, pp. 567-573, Journal Name: Appl. Microbiol., v. 25, no. 4, pp. 567-573; ISSN APMBA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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