STRUCTURAL DEGRADATION OF IRRADIATED PROTEIN

Technical Report · Fri Oct 31 04:00:00 EST 1958

OSTI ID:4328619

Leone, C A; Fricke, H

Irradiated protein systems contain molecules in various stages of structurnl degradation Some of these degraded molecules are insoluble at their isoelectric point and on the basis of the conventional criterion will be described as denatured. Molecules in more moderately injured configrations can be detected since they denature more easily than the native. In order to study -- -these labile molecules quantitatively the denatured constituents of an irradiated system are precipitated and the kinetics of the thermal denaturation of the em to be over the complete range of antigen-antibody combining on and their chemical scussed. An adequate kinetic trons, their reaction stry of the resulting free ics in particle s presented. adiation sensitivity was The exchange of deuterium and B/sup 10/ between diborane and several of the more common boron hydrides was studied because a knowledge of the exchange mechanism gives a better insight into the nature of the intermediate fragments that play an important fole inthe chemistry of these compounds. Evidence has been acumulated for at least three mechanisms by which isotopic exchange proceeds in the boron hydrides, In the diborane selfexchange the reaction appears to involve borane as an entity and the ratio of deuterium rate to boron rate is three. In the B/sub 2/D/sub 6/--B/sub 5/H/sub 9/ exchange the rate-determinin g step is the reaction of borane with thc terminal hydrogens of pentaborane, The bridge hydrogens are not involved at all and the borons do not exchange. A similar situation exists in the diborane-decaborane exchange. IN the B/sub 2/D/sub 6/-B/sub 5/H/sub 11/ reaction the fraction -orde r kinetics indicate that the B/sub 5/H/sub 11/ is fragmenting. All of the borons and all of all of the hydrogens in both molecules are participating in the exchange. Only one rate of exchange has been observed but ther is experimental evidence that exchange can proceed by an intramolecu-lar mechanism. In the case of the B/sub 2/ D/sub 6/-B/sub 4/H/sub 10/ reaction one has at least two mechanisms of isotopic exchange and evidence has been obtained for an exchange that proceeds by an intramolecular mechanism. Kinetic ing. The intramolecular process is an indication of the the borons in some of the less stable boron hydrides. Details of the measurements are discussed for the diborane-tetraborane exange in diborane. The application of some of these results to pyrolysis reactions of boron hydrides is pointed out. (auth)

• Research Organization: Argonne National Lab., Lemont, Ill.; Kansas. Univ., Lawrence
• NSA Number: NSA-12-014648
• OSTI ID: 4328619
• Report Number(s): A/CONF.15/P/859
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDITY
ALBUMINS
AMINO ACIDS
ANTIBODIES
ANTIGENS
BLOOD SERUM
CHEMISTRY
DECOMPOSITION
DETECTION
ENERGY
ENTROPY
EXCITATION
GAMMA RADIATION
IRRADIATION
LIGHT
LOW TEMPERATURE
LYOPHILIZATION
MEASURED VALUES
MOLECULES
OVALBUMIN
PEPTIDES
POLARIZATION
PRECIPITINS
PREPARATION
PROTEINS
PYROLYSIS
QUANTITATIVE ANALYSIS
RABBITS
RADIATION CHEMISTRY
REACTION KINETICS
REPRODUCTION
ROTATION
SEPARATION PROCESSES
SODIUM HYDROXIDES
SOLUBILITY
SOLUTIONS
STABILITY
TEMPERATURE
THERMODYNAMICS
VACUUM