Effect of irradiation on lysosomal activation in HeLa cells

Journal Article · Fri Feb 01 04:00:00 EDT 1974 · Int. J. Radiat. Biol., v. 25, no. 2, pp. 113-120

DOI:https://doi.org/10.1080/09553007414550131· OSTI ID:4325495

Reynolds, C; Wills, E D

The effect of irradiation on the lysosomes of HeLa cells was studied using a new quantitative histochemical method. Up to 5 hrs after gamma irradiation with 5 krads, no activation of the lysosomal enzymes was measured and it was concluded that no immediate damage to the lysosomes had occurred. Twenty- four hours after irradiation with doses of 300 to 1000 rads, lysosomal activation was observed under the light microscope and measured quantitatively; there were increases in lysosomal activity not apparent by observation of gross morphology, and lysosomal enzyme activity increased progressively with dose. It is considered that lysosomal activation is an important consequence of irradiation that contributes to cell damage and death. (auth)

• Research Organization: Saint Bartholomew's Hospital, London
• NSA Number: NSA-29-029675
• OSTI ID: 4325495
• Journal Information: Int. J. Radiat. Biol., v. 25, no. 2, pp. 113-120, Journal Name: Int. J. Radiat. Biol., v. 25, no. 2, pp. 113-120; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*HELA CELLS-- BIOLOGICAL RADIATION EFFECTS
*LYSOSOMES-- BIOLOGICAL RADIATION EFFECTS
CELL KILLING
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
HISTOLOGY
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
TIME DEPENDENCE