Radioassay by Gas Chromatography of Tritium- and Carbon-14-Labeled Compounds

Journal Article · Thu May 01 00:00:00 EDT 1958 · Analytical Chemistry

DOI:https://doi.org/10.1021/ac60137a010· OSTI ID:4324259

Wolfgang, Richard.; Rowland, F. S.

A technique for the simulteneous separation by gas chromatography, mass assay, and radioactivity assay of tritium and carbon-14-labeled organic compounds is described. Helium, which carries the material to be analyzed through the chromatography column and thermal conductivity cell, is converted into a counting gas by the continuous injection of methane. The mixed stream of helium and methane then passes through an internal proportional counter of special design, which detects the radioactivity of the material carried. The scope of this combined technique approaches that of gas chromatography itself. Because the apparatus is simple to build and operate and in view of the increasing availability of labeled compounds, the method should find a wide field of application.

• Research Organization: Yale Univ., New Haven, Conn.; Univ. of Kansas, Lawrence
• Sponsoring Organization: USDOE
• NSA Number: NSA-12-008369
• OSTI ID: 4324259
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 5 Vol. 30; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
CARBON 14
CHROMATOGRAPHY
DETECTION
GASES
HELIUM
INSTRUMENTS
LABELLED COMPOUNDS
LABORATORY EQUIPMENT
MASS
METHANE
MIXING
OPERATION
ORGANIC COMPOUNDS
PROPORTIONAL COUNTERS
RADIOACTIVITY
SEPARATION PROCESSES
THERMAL CONDUCTIVITY
TRACER TECHNIQUES
TRITIUM