ULTRA-VIOLET MICROSCOPY AND CYTOPHOTOMETRY OF NORMAL AND X-IRRADIATED BONE MARROWS

Journal Article · Wed Jan 01 04:00:00 EST 1958 · Biophysics (USSR) (English Translation)

OSTI ID:4294753

Brodskii, V Ya; Suetina, I A

The changes induced by radiation in nucleic acid and nucleotide content of nuclei were studied in bone marrow cells from mice after exposure to 700 r x radiation. Visual studies were made on marrow smears. Characteristics noted included cell and nuclear shape and dimensions, intensity of cytoplasm, and nuclear staining using both basic and acid stains. Unstained cells or tissue sections were studied under the ultraviolet microscope, the images being produced by ultraviolet absorption of the cell materials. Reduced ultraviolet absorption was observed within a few minutes of irradiation, the effect growing stronger with the passage of time. Quantitative cytochemical analysis was made for nucleic acids and nucleotides in the irradiated cells. An analysis of results led to the conclusion that radiation damage to cells is probably reiated to disturbance in nucleic acid metabolism, which causes a decrease in nucleotide content, and not to reduction in amounts of nucleic acid. (C.H.)

• Research Organization: Severtsov Inst. of Animal Morphology, Academy of Sciences of the U.S.S.R., Moscow
• NSA Number: NSA-13-005237
• OSTI ID: 4294753
• Journal Information: Biophysics (USSR) (English Translation), Journal Name: Biophysics (USSR) (English Translation) Vol. Vol: 3
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ABSORPTION
ACIDITY
BIOLOGY AND MEDICINE
BONE MARROW
CYTOLOGY
METABOLISM
MICE
MICROSCOPY
NUCLEIC ACIDS
NUCLEOTIDES
PHOTOMETRY
RADIATION INJURIES
TISSUES
ULTRAVIOLET RADIATION
X RADIATION