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EARLY BIOCHEMICAL REACTIONS FOLLOWING X-IRRADIATION

Technical Report ·
OSTI ID:4293353
;

X-ray-induced changes in biochemical reactions occurring early after irradiation were investigated. Experiments showed that the activity of 12 enzymes of carbohydrate metabolism measured in irradiated liver 20,000 r x rays. After irradiation in purified solution, however, the enzymes showed high radiosensitivity. Further experiments were made on carbohydrate metabolism, especially of tumor cells which were irradiated in vitro. X irradiation with doses of raore than 5,000 r resulted in inhibition of the glycolysis without altering the respiration. Similar effects were observed on carbohydrate metabolism of irradiated slices of spleen, brain, and thymus. The glycolysis of the rat liver remained uninfluenced. By determinations of some metabolite concentrations, the mechanism of the x-rayinduced inhibition of the glycolysis was elucidated. According to these experiments the point of attack of the x-ray effect lies within the glycolysis chain on the step of the dehydrogenation of triosephosphate. The activity of triosephosphate dehydrogenase is not influenced, but it could he demonstrated that the DPN concentration decreases tremendously and will then be limiting for the triosephosphate dehydrogenation. As expected, the inhibition of glycolysis is followed by a decrease of ATP. Consequently, it could be shown that there is an inhibition of C/sup 14/-alanine uptake into the protein of the irradiated tumor cells parallel to the low ATP level. Results indicate that protein synthesis itself is not directly influenced by irradiation. The analysis of free amino acids showed no essential change concerning their concentrations. A clear reduction of the C/sup 14/ activity of the most amino acids was observed with the exception of alanine. (auth)

Research Organization:
Univ.-Frauenklinik, Hamburg-Eppendorf
NSA Number:
NSA-13-006142
OSTI ID:
4293353
Report Number(s):
A/CONF.15/P/994
Country of Publication:
Country unknown/Code not available
Language:
English

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Related Subjects

ADENINE-- ALANINE-- AMINO ACIDS-- ATP-- BIOCHEMISTRY-- CARBOHYDRATES-- CARBON 14-- CHEMICAL REACTIONS-- CYTOLOGY-- DEHYDROGENASES-- ENZYMES-- LABELLED COMPOUNDS-- METABOLISM-- NUCLEIC ACIDS-- ORGANIC PHOSPHORUS COMPOUNDS-- PROTEINS-- QUANTITATIVE ANALYSIS-- QUANTITY RATIO-- RADIATION EFFECTS- - TUMORS-- VARIATIONS-- X RADIATION
BIOCHEMISTRY-- BRAIN-- CARBOHYDRATES-- CYTOLOGY-- ENZYMES-- GLYCOLYSIS-- LIVER-- METABOLISM-- OXIDATION-- RADIATION DOSES-- RADIATION EFFECTS-- RADIOSENSITIVITY-- RATS-- SOLUTIONS-- SPLEEN-- THYMUS-- TUMORS-- X RADIATION
BIOLOGY AND MEDICINE