THE METABOLISM OF ORGANOPHOSPHATES IN CARDIAC TISSUE AS STUDIED WITH RADIOPHOSPHORUS AND PAPER CHROMATOGRAPHY

Technical Report · Sat Oct 31 04:00:00 EST 1959

OSTI ID:4289224

Fleckenstein, A; Janke, J

The metabolism of organophosphates in cardiac tissues was studied using phosphorus-32-labeled orthophosphate. The tracer was administered either to hearts in situ by intravenous injection or by addition to the Ringer bath containing isolated auricles. Cardiac tissue was found to transfer measurable amounts of radiophosphorus from the extracellular space into the intracellular organic phosphorus fractions within a few minutes. To determine the pathway of phosphorus-32 incorporation, heart tissue was frozen in liquid nitrogen at various time intervals. Extracts of frozen muscle powder were analyzed by paper chromatography for activities taken up by the various phosphorus fractions, and the specific activities of phosphorus --molybdate solutions were counted in a fluid counting tube. The data allow an easy calculation of the individual specific activities of the various phosphate groups. Data are tabulated and presented graphically. Results indicate that radiophosphorus and paper chromatography offer a useful tool for studies of cardiac metabolism. (C.H.)

• Research Organization: Univ. of Freiburg, Ger.
• NSA Number: NSA-13-006135
• OSTI ID: 4289224
• Report Number(s): A/CONF.15/P/976
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CHROMATOGRAPHY
COUNTERS
DIAGRAMS
DIFFUSION
HEART
LABELLED COMPOUNDS
LIQUIDS
LOW TEMPERATURE
METABOLISM
NITROGEN
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
QUANTITATIVE ANALYSIS
RADIOACTIVITY
SALTS
SOLIDIFICATION
SOLUTIONS
SOLVENT EXTRACTION
TABLES
TISSUES
TUBES