ANTIGENTIC STIMULI FOR TRANSPLANTATION IMMUNITY TO RAT BONE MARROW HETEROGRAFTS IN LETHALLY X-IRRADIATED MICE

Technical Report · Tue Jul 01 04:00:00 EDT 1958

OSTI ID:4282453

Santos, C W; Cole, L J; Garver, R M

In connection with studies on heterologous bone msrrow transplantation in x-irradiated mice, the question arose as to whether the antigenic stimulus for rejection of such marrow heterografts was solely confined to nuclear fractions as has been reported for skin homografts. Accordingly, LAFm mice were divided into groups of 10 to 15, and injected intraperitoneally 7 to 10 days prior to an LD/ sub 100/ dose of x radiation (870 r) and injection with rat bone marrow, with one of the following suspensions or solutions: rat bone marrow, rat liver, rat red blood cells, non-nuclear iyophilized fraction of rat bone marrow, rat serum, and Tyrode's solution. Blood smears were made on all surviving animals at 7, 14, and 30 days post-irradiation and treated by the alkaline phosphatase technique. All animals injected with Tyrode's solution before irradiation and rat marrow treament showed rat granulocytes (alkaline phosphatase positive) in the peripheral blood at 7, 14, and 30 days. Animals that had been given rat serum prior to irradiation showed no rat cells in the peripheral blood at 7 and 14 days. However, 3 surviving mice at 30 days did show rat granulocytes in their blood. No rat granulocytes were found at one week in the blood of mice which, has received the other treatments. All of these latter mice died before the 14 day. Ten autopsies were performed on mice tom these groups between 8 to 10 days post- irradiation. No rat granulocytes were found in the spleen, liver, lungs or bone marrow of these mice. The data suggest that non-nuclear material may act as one of the direct or indirect stimuli for rejection of a non-solid'' heterograft, in this instance bone marrow cells. (auth)

• Research Organization: Naval Radiological Defense Lab., San Francisco
• NSA Number: NSA-13-000521
• OSTI ID: 4282453
• Report Number(s): USNRDL-TR-254
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANTIGENS
BIOLOGY AND MEDICINE
BLOOD
BLOOD CELLS
BLOOD SERUM
BONE MARROW
CELL NUCLEI
CYTOLOGY
ENZYMES
ERYTHROCYTES
GRANULOCYTES
IMMUNITY
INTESTINE
IRRADIATION
LETHAL DOSE
LEUCOCYTES
LIVER
LUNGS
MICE
PHOSPHATASES
RADIATION DOSES
RATS
SKIN
SOLUTIONS
SPLEEN
SURVIVAL TIME
SUSPENSIONS
TRANSPLANTS
X RADIATION