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Quantitative Studies of Weak Light Sources in Biological Systems by Means of an Image Intensifier

Conference · · IEEE Transactions on Nuclear Science
 [1]
  1. Princeton Univ., NJ (United States). Palmer Physical Laboratory
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Several image intensifier systems have been calibrated for gain and single electron detection efficiency. When these intensifiers are coupled to conventional microscopes the combined system provides sufficient light to allow photographic recording in short exposure times of certain biological systems that would ordinarily not be photographable or would require undesirably long exposure times. The photographs also provide quantitative information on the amount of light entering the microscope objective. Experiments are described in which an image intensifier microscope system has been used to study bioluminescence, providing data on the temporal and spatial distribution of light output in several organisms. Other experiments are described in which the location of weak radioactive tracers has been possible in short exposure times, using thin overlying scintillators to provide the necessary light. System requirements are discussed for the extension of this technique to X-ray studies and fluorescence microscopy.
Research Organization:
Princeton Univ., NJ (United States). Palmer Physical Laboratory
Sponsoring Organization:
US Atomic Energy Commission (AEC)
NSA Number:
NSA-30-034535
OSTI ID:
4268039
Report Number(s):
NYO--3406-7; CONF-680207--9
Conference Information:
Journal Name: IEEE Transactions on Nuclear Science Journal Issue: 3 Journal Volume: 15
Country of Publication:
United States
Language:
English

References (12)

The Relative Sensitivities of Television Pickup Tubes, Photographic Film, and the Human Eye journal June 1942
The breakdown of adenosine triphosphate in the contraction cycle of the frog sartorius muscle journal November 1967
Low-angle X-ray diffraction studies of living striated muscle during contraction journal April 1967
Sensitivity of an Image Intensifier Film System journal April 1966
The Sensitivity Performance of the Human Eye on an Absolute Scale* journal January 1948
Calcium transients in single muscle fibers journal October 1967
The Subcellular Origin of Bioluminescence in Noctiluca miliaris journal May 1967
Scintillomicroscope for Radioactive Tracer Detection journal March 1968
Secondary Electron Multiplication in Image Intensifier Dynode Structures journal January 1966
Evaluation of an Image Intensifier System for Microscopic Observations journal January 1964
Microdetermination of Calcium by Aequorin Luminescence journal June 1963
A method of determining the overall quantum efficiency of visual discriminations journal January 1962

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Related Subjects

*BIOLOGICAL MATERIALS-- LUMINESCENCE
60 APPLIED LIFE SCIENCES
CALIBRATION
EFFICIENCY
ELECTRON DETECTION
FLUORESCENCE
GAIN
IMAGE INTENSIFIERS
MICROSCOPY
PHOTOGRAPHY
RADIOISOTOPES
VISIBLE RADIATION
X RADIATION