Effect of acetaldehyde on Saccharomyces cerevisiae and Zymomonas mobilis subjected to environmental shocks

Journal Article · Sun Jan 05 04:00:00 EST 1997 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/(SICI)1097-0290(19970105)53:1<71::AID-BIT10>3.3.CO;2-I· OSTI ID:419611

Stanley, G A; Hobley, T J; Pamment, N B ^[1]

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1. Univ. of Melbourne, Parkville, Victoria (Australia). Dept. of Chemical Engineering

The lag phase of Saccharomyces cerevisiae subjected to a step increase in temperature or ethanol concentration was reduced by as much as 60% when acetaldehyde was added to the medium at concentrations less than 0.1 g/L. Maximum specific growth rates were also substantially increased. Even greater proportional reductions in lag time due to acetaldehyde addition were observed for ethanol-shocked cultures of Zymomonas mobilis. Acetaldehyde had no effect on S. cerevisiae cultures started from stationary phase inocula in the absence of environmental shock and its lag-reducing effects were greater in complex medium than in a defined synthetic medium. Acetaldehyde reacted strongly with the ingredients of complex culture media. It is proposed that the effect of added acetaldehyde may be to compensate for the inability of cells to maintain transmembrane acetaldehyde gradients following an environmental shock.

• OSTI ID: 419611
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 1 Vol. 53; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ACETALDEHYDE
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
ETHANOL
SACCHAROMYCES CEREVISIAE
YIELDS