Repair of human DNA: radiation chemical damage in normal and xeroderma pigmentosum cells
Conference
·
OSTI ID:4191641
The photolysis of bromodeoxyuridine (BrdUrd) incorporated during repair was used for the assay of DNA repair in human cells. This assay supplies a characterization of the sequence of repair events that occur in human cells after radiation exposure, both ultraviolet and ionizing, and permits an estimation of the size of the average repaired region after these physical insults to DNA. Chemical insults to DNA are discussed and an attempt is made to liken the repair processes after chemical damages of various kinds to those repair processes that occur in human DNA after damage from physical agents. Results are reported indicating that, under certain conditions, repair events resembling those seen after uv radiation can be observed in normal human cells after ionizing radiation and that xeroderma pigmentosum cells, defective in the repair of uv-induced DNA damage, show defective repair of these uv-like DNA lesions induced by ionizing radiation. (CH)
- Research Organization:
- Oak Ridge National Lab., Tenn. (USA); Brookhaven National Lab., Upton, N.Y. (USA)
- NSA Number:
- NSA-33-000893
- OSTI ID:
- 4191641
- Report Number(s):
- CONF-750443--4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
*DNA-- RADIATION INJURIES
560151* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Man
ANIMAL CELLS
BIOASSAY
BIOLOGICAL REPAIR
BUDR
CELL CULTURES
IONIZING RADIATIONS
MAN
N48510* --Life Sciences--Radiation Effects on Animals--Man
PHOTOCHEMISTRY
PHOTOLYSIS
SKIN DISEASES
ULTRAVIOLET RADIATION
560151* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Man
ANIMAL CELLS
BIOASSAY
BIOLOGICAL REPAIR
BUDR
CELL CULTURES
IONIZING RADIATIONS
MAN
N48510* --Life Sciences--Radiation Effects on Animals--Man
PHOTOCHEMISTRY
PHOTOLYSIS
SKIN DISEASES
ULTRAVIOLET RADIATION