UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS

Journal Article · Sat Aug 13 04:00:00 EDT 1960 · Nature

DOI:https://doi.org/10.1038/187614a0· OSTI ID:4180551

Crathorn, A R; Shooter, K V

The uptake of tritium-labelled thymidine by mouse Ehrlich and Landschutz ascites cells in vitro was studied by measuring the activity present in the cell and incorporated into desoxyribonucleic acid (DNA) as a function of time. The distribution of the precursor among the cells was determined by autoradiography. The results show that thymidine is rapidly taken up by cells, but the incorporation rate in DNA is much slower. The precursor studies indicate.that the tritium activity is 15% thymidine di- and tri-phosphates, 25% thymidine monophosphate, and 60% thymidine. The assumption that the activity left in the nucleus after washing and fixing is due to thymidine incorporated into DNA is shown to be invalid; precursors are left behind as well as DNA, and the average activity per cell is about the same for both the two incubation periods and has no relationship to the DNA activity. (D.L.C.)

• Research Organization: Royal Cancer Hospital, London
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-021223
• OSTI ID: 4180551
• Journal Information: Nature, Journal Name: Nature Vol. Vol: 187
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
DISTRIBUTION
LABELLED COMPOUNDS
MEASURED VALUES
METABOLISM
MICE
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE ANALYSIS
RADIOACTIVITY
RADIOAUTOGRAPHY
THYMIDINE
TRITIUM