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Title: UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS

Abstract

The uptake of tritium-labelled thymidine by mouse Ehrlich and Landschutz ascites cells in vitro was studied by measuring the activity present in the cell and incorporated into desoxyribonucleic acid (DNA) as a function of time. The distribution of the precursor among the cells was determined by autoradiography. The results show that thymidine is rapidly taken up by cells, but the incorporation rate in DNA is much slower. The precursor studies indicate.that the tritium activity is 15% thymidine di- and tri-phosphates, 25% thymidine monophosphate, and 60% thymidine. The assumption that the activity left in the nucleus after washing and fixing is due to thymidine incorporated into DNA is shown to be invalid; precursors are left behind as well as DNA, and the average activity per cell is about the same for both the two incubation periods and has no relationship to the DNA activity. (D.L.C.)

Authors:
;
Publication Date:
Research Org.:
Royal Cancer Hospital, London
Sponsoring Org.:
USDOE
OSTI Identifier:
4180551
NSA Number:
NSA-14-021223
Resource Type:
Journal Article
Journal Name:
Nature
Additional Journal Information:
Journal Volume: Vol: 187; Other Information: Orig. Receipt Date: 31-DEC-60
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
BIOLOGY AND MEDICINE; ANIMAL CELLS; DISTRIBUTION; LABELLED COMPOUNDS; MEASURED VALUES; METABOLISM; MICE; NUCLEIC ACIDS; ORGANIC NITROGEN COMPOUNDS; QUANTITATIVE ANALYSIS; RADIOACTIVITY; RADIOAUTOGRAPHY; THYMIDINE; TRITIUM

Citation Formats

Crathorn, A R, and Shooter, K V. UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS. Country unknown/Code not available: N. p., 1960. Web. doi:10.1038/187614a0.
Crathorn, A R, & Shooter, K V. UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS. Country unknown/Code not available. doi:10.1038/187614a0.
Crathorn, A R, and Shooter, K V. Sat . "UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS". Country unknown/Code not available. doi:10.1038/187614a0.
@article{osti_4180551,
title = {UPTAKE OF THYMIDINE AND SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN MOUSE ASCITES CELLS},
author = {Crathorn, A R and Shooter, K V},
abstractNote = {The uptake of tritium-labelled thymidine by mouse Ehrlich and Landschutz ascites cells in vitro was studied by measuring the activity present in the cell and incorporated into desoxyribonucleic acid (DNA) as a function of time. The distribution of the precursor among the cells was determined by autoradiography. The results show that thymidine is rapidly taken up by cells, but the incorporation rate in DNA is much slower. The precursor studies indicate.that the tritium activity is 15% thymidine di- and tri-phosphates, 25% thymidine monophosphate, and 60% thymidine. The assumption that the activity left in the nucleus after washing and fixing is due to thymidine incorporated into DNA is shown to be invalid; precursors are left behind as well as DNA, and the average activity per cell is about the same for both the two incubation periods and has no relationship to the DNA activity. (D.L.C.)},
doi = {10.1038/187614a0},
journal = {Nature},
number = ,
volume = Vol: 187,
place = {Country unknown/Code not available},
year = {1960},
month = {8}
}