STUDIES ON THE DISCRIMINATION AGAINST STRONTIUM BY BONE GROWN IN VITRO

Journal Article · Fri Jul 01 04:00:00 EDT 1960 · Journal of Biological Chemistry (U.S.)

OSTI ID:4179101

Lengemann, F W

Culturing of embryonic bone in vitro showed that both chick and rat bone could discriminate against strontium in favor of calcium. The ends of the bones showed a lower Sr/sup 89//Ca/sup 45/ ratio than the shafts. The degree of selection was not dependent upon the species of origin of the serum used in the medium or upon the age of the bones. Heat-treating of the bones destroyed the ability to select against strontium; for live bone the mechanism functioned at 37 deg but not at 4 deg and 25 deg . Selection occurred at some point after the initial entry of the alkaline earth metals into the bone and was associated with the incorporation of the radioisotopes into a less labile form of bone mineral. Loss of radioactivity from this less labile fraction occurred only with viable bone at 37 deg . The Sr/sup 89/Ca/sup 45/ ratio of the bones relative to the same ratio for the medium as well as the total uptake of radioisotopes could be varied by incorporating enzyme inhibitors into the medium. (auth)

• Research Organization: New York State Veterinary Coll., Ithaca, N.Y.
• NSA Number: NSA-14-017676
• OSTI ID: 4179101
• Journal Information: Journal of Biological Chemistry (U.S.), Journal Name: Journal of Biological Chemistry (U.S.) Vol. Vol: 235; ISSN JBCHA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
BIOLOGY AND MEDICINE
BIRDS
BLOOD SERUM
BONES
CALCIUM 45
CHICKS
EMBRYOS
ENZYMES
METABOLISM
RADIOACTIVITY
RADIOISOTOPES
RATS
STRONTIUM 89
TEMPERATURE
VARIATIONS