Search for biological effects of $sup 13$C-enrichment in developing mammalian systems
Increasing diagnostic use of stable isotopes, especially in children and pregnant women, enhances the importance of studies on the biological isotope effects in sensitive mammalian systems. Experimental data on animal systems are meager. The mouse embryos was studied at various stages and mouse limb buds were studied in organ culture. Limb bud development in vitro was unaffected by incubation with 82 mol percent $sup 13$C-glucose as judged by either morphological or biochemical criteria. Of 271 preimplantation embryos incubated in vitro, 95.2 percent developed normally; in $sup 13$C-enriched medium, 96.5 percent showed normal development. $sup 13$C-Enrichment of the embryos in vitro is over 60 percent. Administration of 1.2 g glucose-U-$sup 13$C to pregnant mice during organogenesis leads to enrichment of maternal liver glycogen to over 17 mol percent $sup 13$C, about one-third this level in the embryo, and a lower level in maternal blood. The absolute $sup 13$C content of the embryo continues to increase for several days after the end of isotope administration, while the enrichment in maternal tissues falls. The lipid fraction of the fetus is most highly labeled shortly after the end of isotope administration. These studies on developing mammalian systems have not yet revealed any alteration of normal development due to stable isotope enrichment. (auth)
- Research Organization:
- Los Alamos Scientific Lab., N.Mex. (USA)
- DOE Contract Number:
- W-7405-ENG-36
- NSA Number:
- NSA-33-001013
- OSTI ID:
- 4173058
- Report Number(s):
- LA-UR-75-1710; CONF-751027-1
- Resource Relation:
- Conference: 2. international conference on stable isotopes, Oak Brook, Illinois, USA, 20 Oct 1975; Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -Biomedical Sciences
Applied Studies-Chemicals Metabolism & Toxicity-Cells
*CARBON 13- ISOTOPE EFFECTS
*EMBRYOS- METABOLISM
*GLUCOSE- METABOLISM
*STABLE ISOTOPES- BIOLOGICAL EFFECTS
*TISSUE CULTURES- METABOLISM
CULTURE MEDIA
IN VITRO
MICE
TISSUE DISTRIBUTION