Localization of radioactivity from 2-methoxy(1,2-14C)ethanol in maternal and conceptus compartments of CD-1 mice

Journal Article · Sun Jun 15 04:00:00 EDT 1986 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(86)90413-8· OSTI ID:5201808

Sleet, R B; John-Greene, J A; Welsch, F

2-Methoxyethanol (ME) induces paw malformations in CD-1 mice when given by gavage on gestation day (gd) 11 (vaginal plug + day = gd 0). The distribution of radioactivity originating from 2-methoxy(1,2-/sup 14/C)ethanol ((/sup 14/C)ME) was examined by liquid scintillation spectrophotometry and whole body autoradiography in pregnant (gd 11) CD-1 mice from 5 min to 48 hr after oral administration. Each dam received either a trace dose of (/sup 14/C)ME (0.92 mumol; 13 muCi) combined with an unlabeled teratogenic dose (187 mumol). By 5 min after the trace dose was administered, /sup 14/C had distributed throughout the maternal and conceptus compartments. Radioactivity in the maternal compartment was most concentrated in the liver, blood and gastrointestinal tract. Conceptus /sup 14/C was associated with the placenta, yolk sac, and embryonal structures such as limb buds, somites, and neuroepithelium. The concentration of blood /sup 14/C plateaued within 30 min after administration of the trace or combined trace/teratogenic dose. It remained stable for 1.5 hr and then gradually declined, reaching 2 to 10% of the maximal concentration by 48 hr. /sup 14/C content in the maternal liver, conceptuses, and embryos per se was always greater than that of the blood and was inversely related to ME dose at 6 hr but not 48 hr. At 6 hr after administration of the trace dose, 69% of total liver and 33% of embryonal /sup 14/C were acid insoluble. Tissue-specific interaction with (/sup 14/C)ME was demonstrated by the distribution of acid insoluble radioactivity among various cellular components of the maternal liver and embryo. The findings indicate that the embryo is readily susceptible to blood borne ME and/or its metabolites. In addition, the chemical characteristics of the labeled molecule(s) apparently favored label incorporation into macromolecules by the liver and embryo.

• Research Organization: Chemical Industry Institute of Toxicology, Research Triangle Park, NC
• OSTI ID: 5201808
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 1; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
ETHANOL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALFORMATIONS
MAMMALS
MICE
NEONATES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PREGNANCY
PRENATAL EXPOSURE
RODENTS
TERATOGENESIS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES