Preparation and chemical characterization of radioiodinated bleomycin

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · J. Nucl. Med., v. 16, no. 9, pp. 835-838

OSTI ID:4172873

Meyers, J; Krohn, K; DeNardo, G

None of the radionuclides with which bleomycin has been labeled have chemical and nuclear properties that are entirely satisfactory for in vivo tumor localization. Bleomycin has been radioiodinated by the iodine monochloride, chloramine-T, and lactoperoxidase methods. Iodine monochloride proved to be the preferred method and conditions were developed whereby 80 percent of radioiodide was covalently bound to bleomycin. Bleomycin (140 $mu$g) was added to 200 $mu$l of saline/citrate buffer (pH 7.0) followed by radioiodide and iodine monochloride. This reaction mixture was incubated for 1 hr and purified by Sephadex G-10 chromatography. The iodine monochloride reaction product underwent hydrolytic deiodination in vitro at a rate of about 1.2 percent/day (0.15 M NaCl, 37$sup 0$C). Bleomycin A and B components were radioiodinated with equal efficiency on a mole fraction basis. (auth)

• Research Organization: Univ. of California, Davis
• NSA Number: NSA-33-001063
• OSTI ID: 4172873
• Journal Information: J. Nucl. Med., v. 16, no. 9, pp. 835-838, Journal Name: J. Nucl. Med., v. 16, no. 9, pp. 835-838; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

*BLEOMYCIN-- LABELLING
*IODINE ISOTOPES-- CHEMICAL BONDS
*RADIOPHARMACEUTICALS-- CHEMICAL PREPARATION
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
N40420 --Chemistry--Radiochemistry & Nuclear Chemistry--Properties of Radioactive Materials
N48830* --Life Sciences--New Tracer Techniques--Other