CHROMATOGRAPHY OF THE PHOSPHOLIPIDES OF RABBIT SKIN

Journal Article · Fri Apr 01 04:00:00 EST 1960 · Archives of Biochemistry and Biophysics (U.S.)

DOI:https://doi.org/10.1016/0003-9861(60)90155-7· OSTI ID:4171023

Schwarz, H P; Dreisbach, L; Stambaugh, R; Kleschick, A; Barrionuevo, M

Mixed lipides of the skin of rabbits have been used for the separation of their phospholipide components on a silicic acid column. Through use of a gradient elution with a number of chloroform-- methanol mixtures and pure methanol, satisfactory separations have been obtained in an 8-hr period. The elution patterns have been constant and the phosphorus recoveries have been high (91%). Combined chemical, infrared, and paper chromaiographic techniques have been used for analysis. Lecithin, phosphatidylethanolamine, and phosphatidylserine comprise the major phospholipide components. Polyglycerolphosphatides, lysolecithin, and sphingomyelin occur in sufficient quantity to be of interest. Small amounts of plasmalogens and traces of two unidentified lipides, one of which appears to be a lysocephalin, make up the rest of the skin phosphatides. (auth)

• Research Organization: Philadelphia General Hospital
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-012458
• OSTI ID: 4171023
• Journal Information: Archives of Biochemistry and Biophysics (U.S.), Journal Name: Archives of Biochemistry and Biophysics (U.S.) Vol. Vol: 87; ISSN ABBIA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CHLOROFORM
CHROMATOGRAPHY
ESTERS
EXTRACTION COLUMNS
INFRARED RADIATION
LECITHINS
LIPIDS
METHANOL
ORGANIC CHLORINE COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PERFORMANCE
PHOSPHOLIPIDS
PHOSPHORIC ACID
PHOSPHORUS
PHOSPHORUS COMPOUNDS
QUALITATIVE ANALYSIS
RABBITS
SEPARATION PROCESSES
SILICIC ACID
SILICON OXIDES
SKIN
SOLVENT EXTRACTION