Preparation of $sup 80$Br or $sup 82$Br-biomolecules via excitation labelling methods

Conference · Wed Jan 01 04:00:00 EST 1975

OSTI ID:4166084

Wong, S; Ache, H J

The direct decay-induced $sup 82$Br (or $sup 80$Br) labelling by exposing the solid substrate molecules, such as deoxyuridine, L-tyrosine, guanosine, deoxycytidine, phenylalanine, and acetic acid, to gaseous CF$sub 3$$sup 82$/sup m/Br (or CF$sub 3$$sup 80$/sup m/Br) was studied. The radiochemical yields of the brominated products are relatively small and range from 1 percent in the case of bromo deoxyuridine to 11 percent for bromoacetic acid. The modification of this technique by adding Cl$sub 2$ gas to the reaction mixture improves the yields in several cases drastically (up to 80 percent for bromo-guanosine and bromo-L-tyrosine). Similar improvement can be achieved by exposing crystalline KBrO$sub 3$ for some time to CF$sub 3$$sup 82$/sup m/Br (or CF$sub 3$$sup 80$/sup m/Br) and dissolving subsequently the KBrO$sub 3$ in an acidic solution of the substrate. (auth)

• Research Organization: Virginia Polytechnic Inst., Blacksburg (USA). Dept. of Chemistry
• NSA Number: NSA-33-002678
• OSTI ID: 4166084
• Report Number(s): CONF-750410--10
• Country of Publication: United States
• Language: English

Related Subjects

*LABELLED COMPOUNDS-- CHEMICAL PREPARATION
*ORGANIC BROMINE COMPOUNDS-- LABELLING
400702* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ACETIC ACID
ALANINES
BROMINATION
CHEMICAL REACTIONS
CHLORINE
DECAY
DEOXYCYTIDINE
GASES
GUANOSINE
ISOMERIC TRANSITIONS
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
NUCLEOSIDES
TYROSINE
URACILS