Radioimmunoassay of plasma testosterone, with use of polyethylene glycol to separate antibody-bound and free hormone

Journal Article · Thu May 01 04:00:00 EDT 1975 · Clin. Chem., v. 21, no. 6, pp. 708-714

OSTI ID:4163333

Anderson, P H; Fukushima, K; Schiller, H S

We have developed a reliable radioimmunoassay for testosterone in plasma, polyethylene glycol (''Carbowax 6000'') being used to separate antibody- bound and free hormone. Testosterone is separated from interfering steroids, notably dihydrotestosterone, by liquid-liquid partition chromatography on infusorial earth (Celite). The assay is sensitive (9 pg for standards), precise, and accurate. The lowest measurable concentration of testosterone is 350 ng/ liter for plasma from men and 70 ng/liter for plasma from women. Intra- and inter-assay coefficients of variation were 6.9 percent and 9.7 percent, respectively, for plasma from men, and 9.6 percent and 11.8 percent, respectively, for plasma from women. Our method for separating antibody-bound and free hormone is practical and convenient and may be generally applicable to all radioimmunoassays of steroid hormones in plasma. (auth)

• Research Organization: Washington School of Medicine, Seattle
• NSA Number: NSA-33-003399
• OSTI ID: 4163333
• Journal Information: Clin. Chem., v. 21, no. 6, pp. 708-714, Journal Name: Clin. Chem., v. 21, no. 6, pp. 708-714; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

*TESTOSTERONE-- RADIOIMMUNOASSAY
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
ACCURACY
ANTIGEN-ANTIBODY REACTIONS
BLOOD PLASMA
CHROMATOGRAPHY
IMMUNE SERUMS
MEN
N48830* --Life Sciences--New Tracer Techniques--Other
POLYETHYLENE GLYCOLS
SENSITIVITY
SEPARATION PROCESSES
TRITIUM COMPOUNDS
WOMEN