Assembly of newly replicated DNA into chromatin

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Cold Spring Harbor Symp. Quant. Biol., v. 38, pp. 247-256

OSTI ID:4162719

Weintraub, H

How does the cell map the ''one-ness'' of the parental chromosome into the ''two-ness'' of its daughters. For the DNA component of the chromosome, the solution, in its most general form, was provided by the realization of the structure of DNA (Watson and Crick, 1953); for the remaining chromosomal elements, very little is known. The experiments to be presented here deal with this question and indicate that the newly synthesized daughter strands become associated with newly synthesized protein within 1 min of replication. Together with recycled protein from the parental chromosome, these two contributions can account for most of the non-DNA mass entering the two daughter chromosomes. During inhibition of protein synthesis, evidence is presented that only one of two daughter DNA strands receives recycled proteins from the parental chromosome. This finding raises the possibility that during normal chromosome replication, the distribution of parental chromosomal proteins to the daughter strands is nondispersive for long stretches of DNA in individual replicons. A preliminary analysis of a new technique for the in vivo cross-linking of proteins to particular regions of the DNA will be described. (auth)

• Research Organization: Lab. of Molecular Biology, Cambridge, Eng.
• NSA Number: NSA-33-007441
• OSTI ID: 4162719
• Journal Information: Cold Spring Harbor Symp. Quant. Biol., v. 38, pp. 247-256, Journal Name: Cold Spring Harbor Symp. Quant. Biol., v. 38, pp. 247-256; ISSN CSHSA
• Country of Publication: United States
• Language: English

Related Subjects

*CHROMATIN-- BIOSYNTHESIS
*DNA-- BIOLOGICAL RADIATION EFFECTS
560121* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Cells--External Source
ANIMAL CELLS
CHICKENS
CHROMOSOMES
DNA REPLICATION
HISTONES
IRRADIATION
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
ULTRAVIOLET RADIATION