Rapid assembly of newly synthesized DNA into chromatin subunits prior to joining of small DNA replication intermediates
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Nuclei from cells having the replicating DNA pulse-labeled with (/sup 3/H) thymidine and the nonreplicating DNA uniformly labeled with (/sup 14/C) thymidine were treated with micrococcal nuclease according to procedures which have been used to study the subunit structure of chromatin. Sedimentation analyses of chromatin from nuclease-treated nuclei, together with measurements of the size of newly synthesized DNA, indicate that (1) chromatin subunits near the replication fork are more susceptible to nuclease attack than subunits in nonreplicating chromatin; (2) newly synthesized DNA is rapidly assembled into chromatin subunits prior to joining of small DNA replication intermediates; and (3) within 10 min after synthesis, DNA in newly replicated chromatin acquires a susceptibility to nuclease treatment similar to that of nonreplicating chromatin.
- Research Organization:
- Los Alamos Scientific Lab., NM
- OSTI ID:
- 7221865
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 73:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL NUCLEI
CHROMATIN
DNA REPLICATION
ENZYMES
HAMSTERS
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MICROCOCCUS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEASES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOTRANSFERASES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SEDIMENTATION
SYNTHESIS
THYMIDINE
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL NUCLEI
CHROMATIN
DNA REPLICATION
ENZYMES
HAMSTERS
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MICROCOCCUS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEASES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOTRANSFERASES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SEDIMENTATION
SYNTHESIS
THYMIDINE
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES