Effect of Basic Dyes on Host-cell Reactivation of Ultra-violet-irradiated Phage
Bacteriophages from bacterial hosts E. coli strains B, B/r, Bs-2, (host-cell reactivation positive-hcr+), and Bs-1 (host-cell reactivation negative-hcr-) were irradiated and then exposed to crystal violet and acriflavine in the plating media. A decrease in the fraction of the damage that can be repaired was found. Presumably the dyes reduce the accessibility of damage regions to the repair enzyme. Besides this effect, the fraction cells that cand repair also decreased. This suggests that bacterial DNA is also necessary for repair and that its action is impaired by the dyes. The results imply that repair by genetic exchandge between phage and bacterial DNA occurs and is mediated by a recombinase enzyme.
- Research Organization:
- Columbia Univ., New York
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-001454
- OSTI ID:
- 4157225
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4903 Vol. 200; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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