Induction of c-myc and c-jun proto-oncogene expression in rat L6 myoblasts by cadmium is inhibited by zinc preinduction of the metallothionein gene
Journal Article
·
· Journal of Toxicology and Environmental Health
- National Cancer Institute, Fredrick, MD (United States)
Certain proto-oncogenes transfer growth regulatory signals from the cell surface to the nucleus. These genes often show activation soon after cells are exposed to mitogenic stimulation but can also be activated as a nonmitogenic stress response. Cadmium (Cd) is a carcinogenic metal in humans and rodents and, though its mechanism of action is unknown, it could involve activation of such proto-oncogenes. Metallothionein (MT), a metal-inducible protein that binds Cd, can protect against many aspects of Cd toxicity, including genotoxicity and possibly carcinogenesis. Thus, the effects of Cd on expression of c-myc and c-jun in rat L6 myoblasts, and the effect of preactivation of the MT gene by Zn treatment on such oncogene expression, were studied. MT protein levels were measured using oligonucleotide hybridization and standardized to {beta}-actin levels. Cd (5 {mu}M CdCl{sub 2}, 0-30 h) stimulated both c-myc and c-jun mRNA expression. An initial peak of activation of c-myc expression occurred 2 h after initiation of Cd exposure, and levels remained elevated throughout the assessment period. Zn pretreatment markedly reduced the activation of c-myc expression by Cd compared to cells not receiving Zn pretreatment. Cd treatment increased c-jun mRNA levels by up to 3.5-fold. Again, Zn pretreatment markedly reduced. 10 refs., 8 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 415116
- Journal Information:
- Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 4 Vol. 48; ISSN 0098-4108; ISSN JTEHD6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transcriptional and post-transcriptional regulation of c-myc, c-myb, and p53 during proliferation and differentiation of murine erythroleukemia cells treated with DFMO and DMSO
Induction of C-FOS, C-MYC and P53 by US -adrenergic receptor (US -AR) stimulation of rat parotid acinar cells (RPAC)
STRAP regulates c-Jun ubiquitin-mediated proteolysis and cellular proliferation
Journal Article
·
Sat Oct 01 00:00:00 EDT 1988
· Experimental Cell Research; (United States)
·
OSTI ID:5464591
Induction of C-FOS, C-MYC and P53 by US -adrenergic receptor (US -AR) stimulation of rat parotid acinar cells (RPAC)
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6359603
STRAP regulates c-Jun ubiquitin-mediated proteolysis and cellular proliferation
Journal Article
·
Fri Apr 08 00:00:00 EDT 2011
· Biochemical and Biophysical Research Communications
·
OSTI ID:22204875