BLOOD CHANGES IN TOTAL-BODY IRRADIATED ALBINO RATS PROTECTED BY N- PHENYLAMIDINE OF THIOPHENE-2-CARBONIC ACID

Journal Article · Sun Jan 01 04:00:00 EST 1961 · Radiobiol. Radiotherap.

OSTI ID:4148506

Zogratov, D; Bayev, I

Changes in peripheral blood cell levels were followed in rats exposed to x-ray doses of 600 r, some of which were pre-treated with 10 mg of this compound, previously shown to be radioprotective in mice. This dose proved absolutely lethal for the unprotected animals, which died between the 10th and 24th day, but 80% of the treated animals survived to at least the 30th day. The onset of anemia was more rapid in the unprotected animals; its maximum extent was reached at the same time (14th-17th day) for both groups, but was higher in the unprotected animals (35% of the initial value), and recovery in this group was delayed. Changes in the reticulocyte count were the same in both groups in all phases; they never disappeared from the blood, the maximum drop occurring on the 3rd to the 5th day and the highest level between the 14th and the 25th day. There were no differences in the leukocyte counts; in both groups leukopenia occurred rapidly (32- 8% of the initial value) on the first day, reached a maximum on the 5th day, after which recovery proceeded slowly on the 17th day, and then rapidly, so that higher than initial values are reached on the 24th day. Changes in the neutrophil count of both groups followed the same trend; the maximum drop occurred on the 5th day, recovery began on the 7th day, reaching values above the initial level on the 24th day. The lymphocyte counts showed no difference between the two groups. There was a sharp drop on the first day, the maximum was reached on the 5th day, recovery began on the 10th day, and on the 25th day the lymphocytes values above the initial ones were reached. Changes in the monocyte count indicated earlier recovery in the protected animals (after the 3rd day); the maximum drop and recovery after the 10th day were the same. The thrombocyte count drop in the protected animals occurred more slowly, its maximum was the same as that of the control group but lower. The results thus confirmed the protective effect of Nphenylamidine of thiophene-2-carbonic acid and that erythropoiesis in the protected animals was less damaged, as manifested by the slower occurrence and lesser degree of anemia and more rapidly recovered. With regard to erythropoiesis and thrombopoiesis the regenerative processes proceeded identically in the two groups. The protective action, therefore, was attributed to repression of the early physicochemical reactions in the irradiated organism and not to the preservation of the regalatory mechanisms which determine the regenerative processes. The more favorable course of the blood changes was considered not as a cause, but as an effect of the lesser damage to the organism as a whole. (BBB)

• Research Organization: Inst. of Radiobiology, Sofia
• NSA Number: NSA-18-001457
• OSTI ID: 4148506
• Journal Information: Radiobiol. Radiotherap., Journal Name: Radiobiol. Radiotherap. Vol. Vol: 2
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMIDINES
ANEMIA
BIOLOGY AND MEDICINE
BLOOD CELLS
BLOOD FORMATION
BODY
CHEMICAL REACTIONS
ERYTHROCYTES
LETHAL DOSE
LEUCOCYTES
LYMPHOCYTES
MONOCYTES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENYL RADICALS
PHYSIOLOGY
PLATELETS
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
RADIATION PROTECTION
RATS
RECOVERY
REGENERATION
RETICULOCYTES
SURVIVAL TIME
VARIATIONS
X RADIATION