Protection of lethally irradiated mice with allogeneic fetal liver cells: influence of irradiation dose on immunologic reconstitution

Journal Article · Wed Oct 01 04:00:00 EDT 1975 · Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4100-4104

DOI:https://doi.org/10.1073/pnas.72.10.4100· OSTI ID:4143589

Tulunay, O; Good, R A; Yunis, E J

After lethal irradiation long-lived, immunologically vigorous C3Hf mice were produced by treatment with syngeneic fetal liver cells or syngeneic newborn or adult spleen cells. Treatment of lethally irradiated mice with syngeneic or allogeneic newborn thymus cells or allogeneic newborn or adult spleen cells regularly led to fatal secondary disease or graft-versus-host reactions. Treatment of the lethally irradiated mice with fetal liver cells regularly yielded long-lived, immunologically vigorous chimeras. The introduction of the fetal liver cells into the irradiated mice appeared to be followed by development of immunological tolerance of the donor cells. The findings suggest that T-cells at an early stage of differentiation are more susceptible to tolerance induction than are T-lymphocytes at later stages of differentiation. These investigations turned up a perplexing paradox which suggests that high doses of irradiation may injure the thymic stroma, rendering it less capable of supporting certain T-cell populations in the peripheral lymphoid tissue. Alternatively, the higher and not the lower dose of irradiation may have eliminated a host cell not readily derived from fetal liver precursors which represents an important helper cell in certain cell-mediated immune functions, e.g., graft-versus-host reactions, but which is not important in others, e.g., allograft rejections. The higher dose of lethal irradiation did not permit development or maintenance of a population of spleen cells that could initiate graft-versus-host reactions but did permit the development of a population of donor cells capable of achieving vigorous allograft rejection. (auth)

• Research Organization: Univ. of Minnesota, Minneapolis
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-012425
• OSTI ID: 4143589
• Journal Information: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4100-4104, Journal Name: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4100-4104; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- IMMUNE REACTIONS
*IMMUNE REACTIONS-- BIOLOGICAL RADIATION EFFECTS
*RADIATION PROTECTION
*SPLEEN CELLS-- IMMUNE REACTIONS
*THYMOCYTES-- IMMUNE REACTIONS
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
DOSE-RESPONSE RELATIONSHIPS
GRAFT-HOST REACTION
LETHAL IRRADIATION
LIVER
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
RADIATIONS