Successful semiallogeneic and allogeneic bone marrow reconstitution of lethally irradiated adult mice mediated by neonatal spleen cells
Spleens of fetal/newborn mice less than 3-4 days of age contain a naturally occurring cell population capable of suppressing T-dependent and T-independent immune responses of third-party adult cells both in vitro and in vivo. We have utilized newborn spleen cells to prevent acute graft-versus-host (GVH) disease in lethally irradiated adult hosts reconstituted with semiallogeneic or even allogeneic bone marrow cells. Pretreatment of reconstituting cell populations with newborn spleen cells reduced the incidence of GVH disease from 100% to 20% in semiallogeneic and from 100% to 40% in allogeneic combinations. Long-term-surviving reconstituted hosts proved immunologically unresponsive to both donor and host histocompatibility antigens, yet possessed a fully chimeric lymphoid system responsive to T and B cell mitogens, as well as unrelated third-party alloantigens.
- Research Organization:
- Univ. of Florida, Gainesville
- OSTI ID:
- 5650102
- Journal Information:
- Transplantation; (United States), Vol. 44:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
TRANSPLANTS
SPLEEN CELLS
GRAFT-HOST REACTION
IMMUNE REACTIONS
LYMPHOCYTES
MICE
RADIATION CHIMERAS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAMMALS
MATERIALS
MOSAICISM
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals