Photodynamic inactivation of antigenic determinants of single-stranded DNA bacteriophage phiX174

Journal Article · Wed May 01 04:00:00 EDT 1974 · J. Virol., v. 13, no. 5, pp. 997-1000

OSTI ID:4143413

Khan, N C; Poddar, R K

Bacteriophage phi X174 when photodynamically inactivated (i.e., when rendered unable to produce plaques as a result of exposure to visible light in air in the presence of proflavine) progressively lost their capacity to bind efficiently with homologous antiserum. Such loss of serum-blocking power was evident with heat-inactivated but not with uv-irradiated phage. The ability of the phages to adsorb to host cells, however, remained practically unaltered even after photodynamic inactivation. It thus appears that photodynamic damages in the so-called ''jacket'' component of the phi X174 coat proteins are partly responsible for the loss of plaque-forming ability, whereas the ''spikes'' are either poor antigens or insensitive to photodynamic treatment. (auth)

• Research Organization: Saha Inst. of Nuclear Physics, Calcutta
• NSA Number: NSA-33-000881
• OSTI ID: 4143413
• Journal Information: J. Virol., v. 13, no. 5, pp. 997-1000, Journal Name: J. Virol., v. 13, no. 5, pp. 997-1000; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

*BACTERIOPHAGES-- BIOLOGICAL RADIATION EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
DNA
INACTIVATION
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOTOCHEMISTRY
ULTRAVIOLET RADIATION