WHOLE-BODY MEASUREMENT OF RADIOACTIVITY AS A MEANS OF FOLLOWING IN VIVO THE DEGRADATION OF I$sup 131$-LABELED PROTEINS IN MICE
Mice were injected with I/sup 181/ -labeled human or bovine serum albumin and determinations made of the rate of loss of radioactivity from the whole body by in vivo counting, from the blood, and from the total trichloroacetic acid precipitable mouse proteins following homogenization. Measurements by each method made more than 8 hours post-injection gave the same half life (14.5 combination rat 0.5 hr) for I/sup 131/ bovine serum albumin (I/ sup 131/BSA) when the level of circulating iodide was sufficient to prevent thyroidal accumulation (I/sup 131/HSA had a half time of 21 hr). The variations observed between the methods in the first 24 hours can be explained in terms of times required for the several compartments to reach secular equilibrium, and therefore whole-body measurements in vivo can be safely used to measure the rate of degradation of this protein when allowance is made for these factors. (auth)
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.
- NSA Number:
- NSA-14-018752
- OSTI ID:
- 4142000
- Journal Information:
- American Journal of Physiology (U.S.), Journal Name: American Journal of Physiology (U.S.) Vol. Vol: 198; ISSN AJPHA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Deferoxamine, a promising bifunctional chelating agent for labeling proteins with gallium: Ga-67 DF-HSA: concise communication
IN VIVO MEASUREMENT BY THE WHOLE-BODY GAMMA SPECTROMETER OF THE DEGRADATION RATE OF I$sup 131$-LABELED NORMAL ALBUMIN