SYNTHESIS OF N'-METHLYNICOTINAMIDE IN X-IRRADIATED RATS

Journal Article · Fri Apr 01 04:00:00 EST 1960 · Intern. J. Radiation Biol.

OSTI ID:4136529

Nerurkar, M K; Sahasrabudhe, M B

The influence of 600 r total-body x-irradiation on the formation and excretion of N' -melhylnicotinarnide (N' MeNA) was investigated. A significant increase in the urinary excretion of N'-MeNA (67.5 per cent) was observed after irradiation. 8tudies of the in vitro N'-MeNA synthesis in liver homogenates revealed that synthetic activity decreased by 19.0 per cent in irradiated rats killed 1 hour later. Addition of ATP or glutathione to the reaction medium could not restore the diminished N'MeNA synthesis, suggesting that the immediate fall in the synthesis of N'-MeNA was probably due to impairment of the enzymes involved in this methylation process. In vitro N'-MeNA synthesis at 20 and 24 hours after x-irradiation, however, was increased by 32.2 and 52.8 per cent respectively. This was corroborated by in vivo studies, which showed 40.5 per cent rise in N'-MeNA synthesis in xirradiated rats. The evidence presented spems to indicate that in addition to the radiochemical degradation of methionine, the acceleration of transmethylation processes may be responsible for the lowering of methionine levels in xirradiated animals. (auth)

• Research Organization: Atomic Energy Establishment, Trombay, India; Indian Cancer Research Centre, Bombay
• NSA Number: NSA-14-016557
• OSTI ID: 4136529
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 2
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ADENINE
AMINO ACIDS
ATP
BIOLOGY AND MEDICINE
BODY
CHEMICAL REACTIONS
ENZYMES
GLUTATHION
IRRADIATION
LIVER
METHIONINE
METHYL RADICALS
NUCLEIC ACIDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRODUCTION
QUANTITY RATIO
RADIATION DOSES
RADIOCHEMISTRY
RATS
TISSUES
URINE
VITAMIN PP
VITAMINS
X RADIATION