MODIFICATION OF RADIATION INDUCED DAMAGE IN BARLEY SEEDS BY THERMAL ENERGY

Journal Article · Wed Jun 01 04:00:00 EDT 1960 · Can. J. Genet. and Cytol.

DOI:https://doi.org/10.1139/g60-013· OSTI ID:4132875

Konzak, C F; Curtis, H J; Delihas, N; Nilan, R A

By freezing seeds of barley, Hordeum vulgare, in dry ice at - 78 deg C, development of increased damage in postradiation storags was inhibited. Frozen irradiated seeds responded to subsequent storage at 23 deg C the same as newly irradiated seed. Preradiation heat treatments of dry seeds reduced observed injury but postradiation heat treatments increased injury. Germination temperature was found to be an important factor determining the level of radiation-induced injury expressed by seeds. Germination at 27 deg C was conducive to lower damage, whereas germination at 20 deg C or lower seemed to favor greater damage. The period of temperature influence on observed injury appeared to be limited, possibly to the duration of the first mitosis in the embryo. Short duration (60 sec) 60 deg C heat shocks on seeds with approximately 16% embryo moisture served not only to reduce injury, measured as seedling height, but also to reduce the frequency of induced chromosome fragments in shoot- tips from irradiated seeds. The results appear to coincide with those obtained following irradiation of biologically important macromolecules such as cellulose, plastics, and DNA. The findings tend to support the thesis that under certain conditions radiation-activated or excited states may mediate a larger or smaller proportion of the total cell damage induced by x radiation. (auth)

• Research Organization: Washington State Univ., Pullman; Brookhaven National Lab., Upton, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-002456
• OSTI ID: 4132875
• Journal Information: Can. J. Genet. and Cytol., Journal Name: Can. J. Genet. and Cytol. Vol. Vol: 2, No. 2
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BOSONS
CELLULOSE
CEREALS
CHROMOSOMES
COMMUTATION RELATIONS
DENSITY
DISTRIBUTION
FOCK REPRESENTATION
GASES
GROUP THEORY
MANY BODY PROBLEM
MATHEMATICS
MATRICES
MECHANICS
MOMENTUM
NUCLEIC ACIDS
NUMERICALS
POLYMERS
QUANTUM MECHANICS
RADIATION INJURIES
SEEDS
STATISTICS
STORAGE
TEMPERATURE
THERMAL RADIATION
VOLUME
X RADIATION