Involvement of Escherichia coli DNA polymerase-I-associated 5' $Yields$ 3' exonuclease in excision-repair of uv-damaged DNA

Conference · Wed Jan 01 00:00:00 EST 1975

OSTI ID:4126145

Heyneker, H; Klenow, H; Hanawalt, P C ^[1]

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1. ed.

From comparative studies between Escherichia coli PolA107 cells (lacking 5' $Yields$ 3' exonucleolytic activity associated with DNA polymerase I) and the isogenic wild-type strain, and between the purified DNA polymerase I preparations isolated from these strains, it can be concluded that the 5' $Yields$ 3' exonuclease is involved in excision of pyrimidine dimers in E. coli. Evidence is presented that the polA107 mutation is located on that part of the DNA polymerase I gene coding for the small fragment on which 5' $Yields$ 3' exonucleolytic activity is found. (auth)

• Research Organization: Leiden State Univ.
• NSA Number: NSA-33-012271
• OSTI ID: 4126145
• Resource Relation: Conference: DNA repair processes conference, Squaw Valley, California, USA, Feb 1974; Other Information: See CONF-740231--PA. Orig. Receipt Date: 30-JUN-76; Related Information: Molecular mechanisms for repair of DNA. Part A
• Country of Publication: United States
• Language: English

Related Subjects

N48310* -Life Sciences-Radiation Effects on Microorganisms- -Basic Studies
N48110 -Life Sciences-Radiation Effects on Biochemicals-In-Vitro
560131* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies
*DNA- BIOLOGICAL RADIATION EFFECTS
*ESCHERICHIA COLI- BIOLOGICAL RADIATION EFFECTS
*NUCLEASES- METABOLISM
*POLYMERASES- METABOLISM
BIOLOGICAL REPAIR
CATALYSIS
MUTATIONS
ULTRAVIOLET RADIATION